Synthesis runs counter to directional folding of a nascent protein domain

Probing nascent protein folding

Deciphering the mechanisms by which proteins fold remains a substantial challenge for structural biology. In particular, very little is known about cotranslational folding, a process in which folding begins as the nascent protein is still emerging from the ribosome. Recognizing the dearth of methods to study this early folding event, Clark and colleagues present a strategy to stall protein tran...

Folding Example Runs to a Workflow Net

We present a folding algorithm to construct a business process model from a specification. The process model is a workflow net, i.e. a Petri net with explicit splitand join-transitions and the specification is a set of example runs. Each example run is a labeled partial order of events, and each event relates to the occurrence of an activity of the underlying business process. In contrast to se...

The ribosome modulates nascent protein folding.

Proteins are synthesized by the ribosome and generally must fold to become functionally active. Although it is commonly assumed that the ribosome affects the folding process, this idea has been extremely difficult to demonstrate. We have developed an experimental system to investigate the folding of single ribosome-bound stalled nascent polypeptides with optical tweezers. In T4 lysozyme, synthe...

US drug strategy runs counter to Canada's.

Folding Partially Ordered Runs

In this paper we present a folding algorithm to construct a process model from a specification given by a set of scenarios. Each scenario is formalized as a partially ordered set of activities of the process. As a process model we consider Event-driven Process Chains which are well established in the domain of business process modeling. Assuming that a specification describes valid behavior of ...